|
Name |
Country |
Label 5’ |
Oligonucleotide Sequence (5’>3’) |
Label 3’ |
Position |
|
|
|
|
N Protein |
|
|
|
2019-nCoV_N1-F |
USA |
|
GACCCCAAAATCAGCGAAAT |
|
28287-28306 |
|
2019-nCoV_N1-R |
|
TCTGGTTACTGCCAGTTGAATCTG |
|
28335-28358 |
|
2019-nCoV_N1-P |
FAM |
ACCCCGCATTACGTTTGGTGGACC |
BQ1 |
28309--28332 |
|
2019-nCoV_N2-F |
|
TTACAAACATTGGCCGCAAA |
|
29164-29183 |
|
2019-nCoV_N2-R |
|
GCGCGACATTCCGAAGAA |
|
29213-29230 |
|
2019-nCoV_N2-P |
FAM |
ACAATTTGCCCCCAGCGCTTCAG |
BQ1 |
29188--29210 |
|
2019-nCoV_N3-F |
|
GGGAGCCTTGAATACACCAAAA |
|
28681-28702 |
|
2019-nCoV_N3-R |
|
TGTAGCACGATTGCAGCATTG |
|
28732-28752 |
|
2019-nCoV_N3-P |
FAM |
AYCACATTGGCACCCGCAATCCTG |
BQ1 |
28706-28727 |
|
2019-nCoV-NF |
China |
|
GGGGAACTTCTCCTGCTAGAAT |
|
28881-28902 |
|
2019-nCoV-NR |
|
CAGACATTTTGCTCTCAAGCTG |
|
28958-28979 |
|
2019-nCoV-NP |
FAM |
TTGCTGCTGCTTGACAGATT |
TAMRA |
28934-28953 |
|
HKU-NF |
Hong Kong |
|
TAATCAGACAAGGAACTGATTA |
|
29145-29166 |
|
HKU-NR |
|
CGAAGGTGTGACTTCCATG |
|
29236-29254 |
|
HKU-NP |
FAM |
GCAAATTGTGCAATTTGCGG |
TAMRA |
29196-29177 |
|
NIID_2019-nCOV_N_F2 |
Japanese |
|
AAATTTTGGGGACCAGGAAC |
|
29142-29161 |
|
NIID_2019-nCOV_N_R2 |
|
TGGCAGCTGTGTAGGTCAAC |
|
29280-29299 |
|
NIID_2019-nCOV_N_P2 |
FAM |
ATGTCGCGCATTGGCATGGA |
BQ1 |
29239-29258 |
|
WH-NIC N-F |
Thailand |
|
CGTTTGGTGGACCCTCAGAT |
|
28320-28339 |
|
WH-NIC N-R |
|
CCCCACTGCGTTCTCCATT |
|
28358-28376 |
|
WH-NIC N-P |
FAM |
CAACTGGCAGTAACCA |
MGB |
28341-38356 |
|
|
|
|
E gene |
|
|
|
E_Sarbeco_F1 |
Germany |
|
ACAGGTACGTTAATAGTTAATAGCGT |
|
26269-26294 |
|
E_Sarbeco_R2 |
|
ATATTGCAGCAGTACGCACACA |
|
26360-26381 |
|
E_Sarbeco_P1 |
FAM |
ACACTAGCCATCCTTACTGCGCTTCG |
BQ1 |
26332-26357 |
|
CoV-EFP |
China |
|
ACTTCTTTTTCTTGCTTTCGTGGT |
|
26295-26318 |
|
CoV-ERP |
|
GCAGCAGTACGCACACAATC |
|
26357-26376 |
|
CoV-EP |
FAM |
CTAGTTACACTAGCCATCCTTACTGC |
BQ1 |
26326-26351 |
|
|
|
|
ORF1ab |
|
|
|
2019-nCoV-OFP |
China |
|
CCCTGTGGGTTTTACACTTAA |
|
13342-13362 |
|
2019-nCoV-ORP |
|
ACGATTGTGCATCAGCTGA |
|
13442-13460 |
|
2019-nCoV-OP |
FAM |
CCGTCTGCGGTATGTGGAAAGGTTATGG |
BQ1 |
13377--13404 |
|
HKU-ORF1b-nsp14F |
Hong Kong |
|
TGGGGYTTTACRGGTAACCT |
|
18778-18797 |
|
HKU- ORF1b-nsp14R |
|
AACRCGCTTAACAAAGCACTC |
|
18889-18909 |
|
HKU-ORF1b-nsp141P |
FAM |
TAGTTGTGATGCWATCATGACTAG |
TAMRA |
18849--18872 |
|
RdRP_SARSr-F2 |
Germany |
|
GTGARATGGTCATGTGTGGCGG |
|
15431-15452 |
|
RdRP_SARSr-R1 |
|
CARATGTTAAASACACTATTAGCATA |
|
15435-15460 |
|
RdRP_SARSr-P2 |
FAM |
CAGGTGGAACCTCATCAGGAGATGC |
BQ1 |
15470-15494 |
|
Name |
|
Label 5’ |
Oligonucleotide Sequence (5’>3’) |
Label 3’ |
|
|
Internal standard |
|
|
RNAse Protein/NM_006413.5 |
|
|
|
RP-F |
Recognized |
|
AGATTTGGACCTGCGAGCG |
|
28-46 |
|
RP-R |
|
GAGCGGCTGTCTCCACAAGT |
|
73-92 |
|
RP-P RNAse P |
FAM |
TTCTGACCTGAAGGCTCTGCGCG |
BQ1 |
49-71 |
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