Sample
Requirements 1.Cells
(in vitro cultured cells or white blood cells, purified
peripheral blood mononuclear cells (PBMCs): One to 5
million cells. When FACS-sorted and highly purified
cells are provided, the number may be as low as 100,000
cells. We have performed TaqMan®
analysis for gene transcription using blastocysts at the
32 and 64-cell stage with excellent results.The enough
harvested cells which be stored in Trizol®
could be shipped frozen
(-20¡ãC) in styrofoam boxes.The
styrofoam boxes should be recycled. 2.Tissues: Fresh tissue should be provided in a
frozen state on dry ice (stored at-80¡ãC for up to 1
year). Please provide tissue samples at least 50-100
mg.The grinded sample which be stored in Trizol®
could be shipped frozen
(-20¡ãC) in styrofoam boxes.The
styrofoam boxes should be recycled. 3.Fixed tissues: Two 50 micron sections of
paraffin-embedded tissues are required from solid
tissues. A third 50 micron section is required from
non-solid tissues such as lung tissue. TaqMan®
analysis were performed on a variety of
paraffin-embedded tissues (i.e. skin, lymph node, GI
tract, spleen, liver, lung, brain) which were up to 20
years of age without significant loss of PCR signals for
the endogenous control (GAPDH,beta-atin or 18S rRNA)
when compared to recently embedded tissue (within 1
year).
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