Q1:
How can I getfree
samples?
A1: ShineGene would be glad to provide some free samples
to our clients only if you have a FedEx, DHL, TNT ,UPS
account or other courier freight collectable account or
agree to pre-pay the freight. What you need do is to
tell us the Cat. No. and courier freight collectable
account via email or fax, and ShineGene will arrange for
shipment as soon as we can.
Q2:How can I get your details of products and price
information?
A2: Please contact us via email or fax to tell us your
requirement. ShineGene will reply to you as soon as
possible, including all information such as product
description and price quote.
Q3:How
can I place an order?
A3:
- for an order for services:
1. Please send us your inquiry of services in detail via
email or fax.
2. ShineGene will reply to you per your inquiry
including price and delivery time.
3. You need confirm our price or delivery time after you
receive our feedback.
4.
ShineGene will begin the service,as soon as we received
your confirmation. -
for an order for products:
Please send us your purchase order with signature and
company stamps by email or fax. You can also ask us to
send you a proforma invoice for your order, and in order
to do this, we need to know the following information
for your order:
* Shipping information, including company name, address,
phone number, fax number,
destination airport or sea port, transportation method (
by courier, by air or by ocean liner).
* Product information, including product numbers,
product name and quantity.
* Delivery time.
* Freight payment ( by collect or pre-pay )
* Forwarder's contact details ( in China)
Q4:
How will the products be sent to us?
A4: Normally, ShineGene use FedEx or UPS to ship our
products. For international customers, delivery charge
is based on the quantity and products to be shipped as
per different cases. Please inquire for prices on
International shipments. All ShineGene¡¯s biological
products are stored and shipped frozen (-20¡ãC) in
styrofoam boxes. The dry ice inside the box will be able
to hold the product¡¯s temperature below -20¡ãC for 3
days at an ambient temperature of 25¡ãC. To avoid waste
and protect environment, the styrofoam boxes should be
recycled.
Q5:
What's the warranty of ShineGene¡¯s products?
A5: ShineGene warrant that all our products will meet
the specification stated on the technical data sheet and
agree to replace the product free of charge if it does
not conform to the specifications. But your notice for
replacement must be given within 30 days after receiving
it.
Q6:
What's ShineGene¡¯s usual payment term for orders?
A6: Please be noted that we don't accept credit card.
ShineGene only accept wire transfer ( T/ T) to our bank
account after order confirmation.
ShineGene have different payment terms for different
customers.
For a contracted customer, we grant payment against
delivery; for a new customer, we need 100%
prepayment; and for a regular one, we ask for 30%
prepayment and 70% delivery against payment.
You will be informed of payment information (bank
address and account data) on each invoice. The invoice
will be sent to you by email to save time and money. In
order to shorten the delivery time, please fax or email
us a copy of the wire transfer once you settle the
payment, so our production will start after receiving
the copy, and the delivery will be made after your
payment arrival.
Q7:
How can I be your sole distributor?
A7: If you can sell our products or services by USD5,000
to USD30,000 per month, you could be one of our
distributor in your city or area; if you can sell our
products by more than USD50,000 per month, you could be
our sole distributor in the country.
Q8:What
is the advantage of working with SYBRGreen
I?
A8:SYBR Green I is an inexpensive, universal dye which
binds to all dsDNA. It can be easily used in combination
with a simple primer pair to detect PCR products in
Real-Time. This dye is mainly very attractive for
researchers analysis lots of different genes. However it
is important to do a good primer design to avoid primer
dimers, which will also be detected by SYBR Gre
en I.
Q9:What
is the advantage of working with a probe system?
A9:A probe system is always specific (except Amplifluorprobes)
and therefore does only
detect the gene of interest. If you have a difficult to
optimise PCR it will not show you any
primer dimers or aspecific products.With a probe system
it is also possible to distinguish between similar
sequences with small differences like SNPs or
mutations.In general, probe assays need less
optimisation than SYBRGreen
I assays.
Q10:Is
a probe assay more sensitive than a SYBRGreen
I assay?
A10:A
probe assay and a SYBRGreen
I assay can be equally sensitive. In cases of difficult
to optimise PCRs the SYBRGreen
I assay might be less beneficial as it shows the total
fluorescent signal of primer dimers, aspecific product
and wanted product.
Q11:Can
I use a SYBR® Green I kit for a probe assay?
A11:This
is not possible because the buffer for SYBRGreen
I kits and the buffer for probe kits are different. The
kits for SYBRGreen
I applications contain special stabilizers to prevent
the SYBRGreen
I from degradation and do differ in pH and salt
concentration.
A12:How
should the qPCR and RT qPCR kits be stored?
A12:All
qPCR kits should be stored at ¨C20¡ãC
and are guaranteed stable for 2 years. Mastermixes can
also be stored at 4¡ãC
for one month. One step RT qPCR kits should be stored at
¨C20¡ãC
and are guaranteed stable for six months. Two step RT
qPCR kits should be stored preferably at ¨C70¡ãC
and are guaranteed stable for six months.
Q13:What
is absolute quantitation?
A13:Absolute
quantitation is a method in which unknown samples are
quantitated by comparing them to a standard curve, based
on absolute amounts of DNA.
Q14:What
is relative quantitation?
A14:Relative
quantitation is a method in which unknown samples are
compared to reference samples to determine the increase
or decrease of gene expression. The out come is
presented as an index, not an absolute amount.
Q15:How
should qPCR probes be stored?
A15:After
dissolving qPCR probes they should be aliquot and stored
at ¨C20¡ãC
in the dark and undergo not more than 5 freeze-thaw
cycles.
Q16:How
many reactions can be done with one synthesis of
Double-Dye Oligo?
A16:One
batch of Double-Dye Oligo, produced in 0,2 ¦Ìmol
scale, will give a final yield of minimal 10 mol. This
is on average sufficient for 2000 qPCR reactions of 50 ¦Ìl.
Q17:How
can a low signal to noise ratio for Molecular Beacons be
explained?
A17:When
designing Molecular Beacons, pay attention to the
folding of the probe. It might fold into alternate
conformations, which are not well quenched. Change the
stem or loop sequence, or both to avoid this.If the salt
concentration of the buffer is too low (below 1 mM
MgCl2) the probe does alsonot
fold correctly.Choose Eurogentec¡¯s Molecular Beacons
and we will help you with the design.
¡¡
Copyright 2003-2007 Shanghai ShineGene Molecular Biotech,Inc. All Rights
Reserved.
Floor 2,Building A,328#, Wuhe Road,Minhang District,Shanghai,201109,China
Tel:0086-21-54460832 Fax:0086-21-54460831-13
E-mail:master@shinegene.org.cn